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AIM: To study the expression of c-fos mRNA in brain following moderate lateral fluid percussion brain injury in rats, and to observe the temporal patterns of its expression following percussion. METHODS: Male Sprague-Dawley rats were divided into normal control, sham operation control and injury group. The rats of injury group subjected to moderate lateral fluid percussion injury (0.2 mPa). The injury groups were then subdivided into 5 min, 15 min, 30 min, 1h, 2h groups according to the time elapsed after injury. The expression of c-fos mRNA was studied with reverse transcription polymerase chain reaction (RT-PCR) semi-quantitatively.RESULTS: At 5 min after percussion, the induction of c-fos mRNA was increased, and remained elevated up to 2 h after brain injury.CONCLUSION: The induction and expression of the c-fos mRNA in cortex and brain stem after fluid percussion brain injury were increased rapidly.
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AIM: To study the expression of c-jun in brain stem following moderate lateral fluid percussion brain injury in rats, and to observe the temporal patterns of its expressions following percussion.METHODS: Male Sprague-Dawley rats were divided into normal control, sham operation control and injury groups. The rats of injury group subjected to moderate lateral fluid percussion injury (0.2 mPa), and then were subdivided into 5 min, 15 min, 30 min, 1 h, 2 h, 4 h, 8 h and 12 h groups according to the time elapsed after injury. The expression of c-jun was studied by immunohistochemistry and in situ hybridization. RESULTS: After percussion for 15 min, Jun positive neurons increased in brain stem progressively, and peaked at 12h. At 5min after percussion, the induction of c-jun mRNA was increased, and remained elevated up to 1h-2h after brain injury. CONCLUSION: The induction and expression of the c-jun in brain stem after fluid percussion brain injury were increased rapidly and lasted for a long time.
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Objective To develop a software for communication interface of laboratory analyzer for single-way or two-way communication of laboratory analyzer so as to ensure the data accuracy.Methods Based on PowerBuilder 9.0,MSCOMM.OCX was programmed to connect PC and the laboratory analyzer by right settings of communication parameters.Results All data were received stably and accurately without errors in running.This software replaced manual recording absolutely.Conclusion The communication interface software for laboratory analyzers not only realizes automatic data acquisition to the greatest extend and ensures the accuracy,but also makes the laboratory working procedure more reasonable and data management more standardized.
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Objective To analyze the application of bar code mode in LIS and help select a fit bar code mode for the hospital. Methods The mode of print bar code was chosen on the base of LIS that was programmed with ORACLE database and PowerBuider9.0 so as to manage laboratory information. Results The bar code management in LIS was realized and no- paper thoroughly in hospital. Conclusion The print bar code mode is more suitable for information-based hospital.
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A case of the Sick Sinus Syndrome which was based on the diagnosis ofautopsy is reported in this paper.It was found that a decreased number ofpacemaker cells with fibrosis,fatty infiltration and the thick endothelium ofcenter arteriole in sinus-atrial node.Otherwise the vacuolar degenerationcould be seen in the bundle of his.There were no fetal pathological changesto be seen in other organs.